The Journal of cell biology, 2007-01, Vol.176 (2), p.141-146
2007
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Details
- Autor(en) / Beteiligte
- Titel
- AAA⁺ protein ATAD3 has displacement loop binding properties and is involved in mitochondrial nucleoid organization
- Ist Teil von
- The Journal of cell biology, 2007-01, Vol.176 (2), p.141-146
- Ort / Verlag
- United States: The Rockefeller University Press
- Erscheinungsjahr
- 2007
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Many copies of mammalian mitochondrial DNA contain a short triple-stranded region, or displacement loop (D-loop), in the major noncoding region. In the 35 years since their discovery, no function has been assigned to mitochondrial D-loops. We purified mitochondrial nucleoprotein complexes from rat liver and identified a previously uncharacterized protein, ATAD3p. Localization studies suggested that human ATAD3 is a component of many, but not all, mitochondrial nucleoids. Gene silencing of ATAD3 by RNA interference altered the structure of mitochondrial nucleoids and led to the dissociation of mitochondrial DNA fragments held together by protein, specifically, ones containing the D-loop region. In vitro, a recombinant fragment of ATAD3p bound to supercoiled DNA molecules that contained a synthetic D-loop, with a marked preference over partially relaxed molecules with a D-loop or supercoiled DNA circles. These results suggest that mitochondrial D-loops serve to recruit ATAD3p for the purpose of forming or segregating mitochondrial nucleoids.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9525eISSN: 1540-8140DOI: 10.1083/jcb.200609158Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2063933
- Format
- –
- Schlagworte
- Adenosine Triphosphatases, Adenosine Triphosphate - metabolism, Animals, ATPases Associated with Diverse Cellular Activities, Binding Sites, Binding, Competitive, Cell Line, Tumor, DNA, DNA, Mitochondrial - genetics, DNA, Mitochondrial - metabolism, DNA, Single-Stranded - metabolism, DNA, Superhelical - genetics, DNA, Superhelical - metabolism, DNA-Binding Proteins - genetics, DNA-Binding Proteins - metabolism, Electrophoresis, Gel, Two-Dimensional, Electrophoretic Mobility Shift Assay, Gels, Humans, Liver, Medical research, Membrane Proteins - genetics, Membrane Proteins - metabolism, Mitochondria, Mitochondria, Liver - metabolism, Mitochondrial DNA, Mitochondrial Proteins - genetics, Mitochondrial Proteins - metabolism, Molecules, Nucleic Acid Conformation, Nucleoproteins - genetics, Nucleoproteins - metabolism, Peptide Fragments - metabolism, Plasmids, Plasmids - metabolism, Protein Binding, Proteins, Rats, RNA, Small Interfering - genetics, Rodents, Small interfering RNA, Submitochondrial Particles - metabolism