Biochemical and biophysical research communications, 2007-10, Vol.362 (1), p.132-138
2007
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- Autor(en) / Beteiligte
- Titel
- RAP80 interacts with the SUMO-conjugating enzyme UBC9 and is a novel target for sumoylation
- Ist Teil von
- Biochemical and biophysical research communications, 2007-10, Vol.362 (1), p.132-138
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2007
- Quelle
- Elsevier ScienceDirect Journals
- Beschreibungen/Notizen
- RAP80, a nuclear protein with two functional ubiquitin-interaction motifs (UIMs) at its N-terminus, plays a critical role in the regulation of estrogen receptor alpha and DNA damage response signaling. A yeast two-hybrid screen identified the SUMO-conjugating enzyme UBC9 as a protein interacting with RAP80. The interaction of RAP80 with UBC9 was confirmed by co-immunoprecipitation and GST pull-down analyses. The region between aa 122–204 was critical for the interaction of RAP80 with UBC9. In addition, we demonstrate that RAP80 is a target for SUMO-1 modification in intact cells. Expression of UBC9 enhanced RAP80 mono-sumoylation and also induced multi-sumoylation of RAP80. In addition to SUMO-1, RAP80 was efficiently conjugated to SUMO-3 but was only a weak substrate for SUMO-2 conjugation. These findings suggest that sumoylation plays a role in the regulation of RAP80 functions.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-291XeISSN: 1090-2104DOI: 10.1016/j.bbrc.2007.07.158Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2049087
- Format
- –
- Schlagworte
- Amino Acid Motifs, Amino Acid Sequence, Carrier Proteins - metabolism, Carrier Proteins - physiology, DNA Damage, DNA DAMAGES, DNA-Binding Proteins, ENZYMES, Estrogen Receptor alpha - metabolism, ESTROGENS, GLUTATHIONE, HeLa Cells, Histone Chaperones, Humans, IONIZING RADIATIONS, Molecular Sequence Data, Nuclear Proteins - metabolism, Nuclear Proteins - physiology, Protein Structure, Tertiary, Radiation, Ionizing, RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS, RAP80, RECEPTORS, Signal Transduction, SUMO-1, SUMO-1 Protein - metabolism, Sumoylation, Two-Hybrid System Techniques, UBC9, Ubiquitin - chemistry, Ubiquitin - metabolism, Ubiquitin-Conjugating Enzymes - physiology, YEASTS