The American journal of pathology, 2000-03, Vol.156 (3), p.965-976
2000
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- Autor(en) / Beteiligte
- Titel
- Hypoxia-Induced Vascular Endothelial Growth Factor Expression Precedes Neovascularization after Cerebral Ischemia
- Ist Teil von
- The American journal of pathology, 2000-03, Vol.156 (3), p.965-976
- Ort / Verlag
- Bethesda, MD: Elsevier Inc
- Erscheinungsjahr
- 2000
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We investigated the hypothesis that hypoxia induces angiogenesis and thereby may counteract the detrimental neurological effects associated with stroke. Forty-eight to seventy-two hours after permanent middle cerebral artery occlusion we found a strong increase in the number of newly formed vessels at the border of the infarction. Using the hypoxia marker nitroimidazole EF5, we detected hypoxic cells in the ischemic border of the neocortex. Expression of vascular endothelial growth factor (VEGF), which is the main regulator of angiogenesis and is inducible by hypoxia, was strongly up-regulated in the ischemic border, at times between 6 and 24 hours after occlusion. In addition, both VEGF receptors (VEGFRs) were up-regulated at the border after 48 hours and later in the ischemic core. Finally, the two transcription factors, hypoxia-inducible factor-1 (HIF-1) and HIF-2, known to be involved in the regulation of VEGF and VEGFR gene expression, were increased in the ischemic border after 72 hours, suggesting a regulatory function for these factors. These results strongly suggest that the VEGF/VEGFR system, induced by hypoxia, leads to the growth of new vessels after cerebral ischemia. Exogenous support of this natural protective mechanism might lead to enhanced survival after stroke.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0002-9440eISSN: 1525-2191DOI: 10.1016/S0002-9440(10)64964-4Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1876841
- Format
- –
- Schlagworte
- Animals, Biological and medical sciences, Brain Ischemia - complications, Brain Ischemia - metabolism, Brain Ischemia - pathology, Disease Models, Animal, DNA - analysis, DNA Primers - chemistry, DNA-Binding Proteins - metabolism, Endothelial Growth Factors - genetics, Endothelial Growth Factors - metabolism, Hypoxia - complications, Hypoxia - metabolism, Hypoxia - pathology, Hypoxia-Inducible Factor 1, Hypoxia-Inducible Factor 1, alpha Subunit, Immunohistochemistry, In Situ Hybridization, Ki-67 Antigen - metabolism, Lymphokines - genetics, Lymphokines - metabolism, Medical sciences, Mice, Neovascularization, Pathologic - etiology, Neovascularization, Pathologic - metabolism, Neovascularization, Pathologic - pathology, Neurology, Nuclear Proteins - metabolism, Peptide Initiation Factors - metabolism, Platelet Endothelial Cell Adhesion Molecule-1 - metabolism, Proto-Oncogene Proteins - genetics, Proto-Oncogene Proteins - metabolism, Receptor Protein-Tyrosine Kinases - genetics, Receptor Protein-Tyrosine Kinases - metabolism, Receptors, Growth Factor - genetics, Receptors, Growth Factor - metabolism, Receptors, Vascular Endothelial Growth Factor, Regular, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger - metabolism, Transcription Factors - biosynthesis, Up-Regulation, Vascular diseases and vascular malformations of the nervous system, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factor Receptor-1, Vascular Endothelial Growth Factors