Journal of clinical pathology, 2006-02, Vol.59 (2), p.179-183
2006
Details
- Autor(en) / Beteiligte
- Titel
- Caspase activity is downregulated in choriocarcinoma: a cDNA array differential expression study
- Ist Teil von
- Journal of clinical pathology, 2006-02, Vol.59 (2), p.179-183
- Ort / Verlag
- London: BMJ Publishing Group Ltd and Association of Clinical Pathologists
- Erscheinungsjahr
- 2006
- Link zum Volltext
- Quelle
- BMJ Journals Archiv - DFG Nationallizenzen
- Beschreibungen/Notizen
- Background: Placental trophoblast can be considered to be pseudomalignant tissue and the pathogenesis of gestational trophoblastic diseases remains to be clarified. Aims: To examine the role of caspases 8 and 10, identified by differential expression, on trophoblast tumorigenesis. Methods: cDNA array hybridisation was used to compare gene expression profiles in choriocarcinoma cell lines (JAR, JEG, and BeWo) and normal first trimester human placentas, followed by confirmation with quantitative real time polymerase chain reaction and immunohistochemistry. Caspase 10 and its closely related family member caspase 8 were analysed. Results: Downregulation of caspase 10 in choriocarcinoma was detected by both Atlas™ human cDNA expression array and Atlas™ human 1.2 array. Caspase 10 mRNA expression was significantly lower in hydatidiform mole (p = 0.035) and chorioarcinoma (p = 0.002) compared with normal placenta. The caspase 8 and 10 proteins were expressed predominantly in the cytotrophoblast and syncytiotrophoblast, respectively, with significantly lower expression in choriocarcinomas than other trophoblastic tissues (p < 0.05). Immunoreactivity for both caspase 8 and 10 correlated with the apoptotic index previously assessed by terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (p = 0.02 and p = 0.04, respectively) and M30 (p < 0.001 and p = 0.003, respectively) approaches. Conclusions: These results suggest that the downregulation of capases 8 and 10 might contribute to the pathogenesis of choriocarcinoma.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9746eISSN: 1472-4146DOI: 10.1136/jcp.2005.028027Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1860314
- Format
- –
- Schlagworte
- Age, Apoptosis, Biological and medical sciences, Caspase 10, Caspase 8, caspases, Caspases - biosynthesis, Caspases - genetics, CCA, cDNA array, Cell cycle, Chemotherapy, choriocarcinoma, Choriocarcinoma - enzymology, Choriocarcinoma - pathology, Deoxyribonucleic acid, Disease, DNA, DNA, Neoplasm - genetics, Down-Regulation, Experiments, Female, Gene expression, Gene Expression Profiling - methods, Gene Expression Regulation, Neoplastic, gestational trophoblastic disease, gestational trophoblastic neoplasia, GTD, GTN, hCG, human chorionic gonadotrophin, Humans, hydatidiform mole, Hydatidiform Mole - enzymology, Hydatidiform Mole - pathology, Investigative techniques, diagnostic techniques (general aspects), Medical sciences, Metastasis, Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis, Original, Pathogenesis, Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques, PCR, Placenta - enzymology, polymerase chain reaction, Pregnancy, Real time, Reverse Transcriptase Polymerase Chain Reaction - methods, reverse transcription, Studies, Suctioning, terminal deoxynucleotidyl transferase mediated dUTP nick end labelling, Tumor Cells, Cultured, TUNEL, Uterine Neoplasms - enzymology, Uterine Neoplasms - pathology