FEBS letters, 2007-03, Vol.581 (5), p.949-954
2007
Details
- Autor(en) / Beteiligte
- Titel
- Neurogenin 3 recruits CBP co-activator to facilitate histone H3/H4 acetylation in the target gene INSM1
- Ist Teil von
- FEBS letters, 2007-03, Vol.581 (5), p.949-954
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 2007
- Link zum Volltext
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- INSM1 is a downstream target gene of neurogenin 3 (ngn3). A promoter construct containing the −426/+40 bp region transiently co-transfected into NIH-3T3 cells with a ngn3 expression plasmid resulted in a 12-fold increase in promoter activity. The ngn3/E47 heterodimer selectively binds and activates the E-box3 of the INSM1 promoter. The endogenous ngn3 and CREB-binding protein (CBP) co-activator occupy the INSM1 promoter, resulting in hyper-acetylation of histone H3/H4 chromatin in a human neuroblastoma cell line, IMR-32. Additionally, adenoviral ngn3 can induce endogenous INSM-1 expression in pancreatic ductal carcinoma-1 cells through the recruitment of CBP to the INSM1 promoter and increase the acetylation of the INSM1 promoter region.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/j.febslet.2007.01.087Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1839826
- Format
- –
- Schlagworte
- Acetylation, Animals, Base Sequence, basic helix-loop-helix, Basic Helix-Loop-Helix Transcription Factors - genetics, Basic Helix-Loop-Helix Transcription Factors - metabolism, bHLH, Carrier Proteins - genetics, Carrier Proteins - metabolism, CAT, CBP, Cell Line, ChIP assay, chloramphenicol acetyltransferase, chromatin immunoprecipitation assay, Corticosterone, CREB-binding protein, DNA Primers - genetics, DNA-Binding Proteins - genetics, electrophoretic mobility shift assay, EMSA, Gene Expression Regulation, Histone acetylation, Histones - chemistry, Histones - metabolism, Humans, INSM1, Insulinoma-associated 1, Mice, Nerve Tissue Proteins - genetics, Nerve Tissue Proteins - metabolism, Neurogenin 3, ngn3, NIH 3T3 Cells, PANC-1, pancreatic ductal carcinoma, Promoter Regions, Genetic, Recombinant Proteins - genetics, Recombinant Proteins - metabolism, Repressor Proteins - genetics, Transcription Factors - genetics, Transcriptional activation, Transfection