The Plant cell, 2003-09, Vol.15 (9), p.2093-2105
2003
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Details
- Autor(en) / Beteiligte
- Titel
- Endoreduplication Mediated by the Anaphase-Promoting Complex Activator CCS52A Is Required for Symbiotic Cell Differentiation in Medicago truncatula Nodules
- Ist Teil von
- The Plant cell, 2003-09, Vol.15 (9), p.2093-2105
- Ort / Verlag
- United States: American Society of Plant Biologists
- Erscheinungsjahr
- 2003
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- In Medicago nodules, endoreduplication cycles and ploidy-dependent cell enlargement occur during the differentiation of bacteroid-containing nitrogen-fixing symbiotic cells. These events are accompanied by the expression of ccs52A, a plant ortholog of the yeast and animal cdh1/srw1/fzr genes, acting as a substrate-specific activator of the anaphase-promoting complex (APC) ubiquitin ligase. Because CCS52A is involved in the transition of mitotic cycles to endoreduplication cycles, we investigated the importance of somatic endoploidy and the role of the M. truncatula ccs52A gene in symbiotic cell differentiation. Transcription analysis and ccs52A promoter-driven β-glucuronidase activity in transgenic plants showed that ccs52A was dispensable for the mitotic cycles and nodule primordium formation, whereas it was induced before nodule differentiation. The CCS52A protein was present in the nucleus of endoreduplication-competent cells, indicating that it may activate APC constitutively during the endoreduplication cycles. Downregulation of ccs52A in transgenic M. truncatula plants drastically affected nodule development, resulting in lower ploidy, reduced cell size, inefficient invasion, and the maturation of symbiotic cells, accompanied by early senescence and finally the death of both the bacterium and plant cells. Thus, ccs52A expression is essential for the formation of large highly polyploid symbiotic cells, and endoreduplication is an integral part of normal nodule development.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1040-4651eISSN: 1532-298XDOI: 10.1105/tpc.014373Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_181333
- Format
- –
- Schlagworte
- Alfalfa, Anaphase-Promoting Complex-Cyclosome, Apoptosis, Apoptosis - physiology, Bacteroids, Cell cycle, Cell Cycle Proteins, Cell Cycle Proteins - genetics, Cell Cycle Proteins - metabolism, Cell Differentiation, Cell Differentiation - genetics, Cell Differentiation - physiology, Cell Division, Cell Division - genetics, Cell Division - physiology, Cell growth, Cellular senescence, Cyclins, Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Glucuronidase, Glucuronidase - genetics, Glucuronidase - metabolism, Life Sciences, Medicago, Medicago - genetics, Medicago - microbiology, Medicago - ultrastructure, Meristems, Microscopy, Electron, Nitrogen Fixation, Nitrogen Fixation - genetics, Nitrogen Fixation - physiology, Nodules, Plant cells, Plant Proteins, Plant Proteins - genetics, Plant Proteins - metabolism, Plants, Plants, Genetically Modified, Polyploidy, Sinorhizobium meliloti, Sinorhizobium meliloti - growth & development, Sinorhizobium meliloti - ultrastructure, Symbiosis, Symbiosis - genetics, Symbiosis - physiology, Transgenic plants, Ubiquitin-Protein Ligase Complexes, Ubiquitin-Protein Ligase Complexes - genetics, Ubiquitin-Protein Ligase Complexes - metabolism, Ubiquitin-Protein Ligases, Ubiquitin-Protein Ligases - genetics, Ubiquitin-Protein Ligases - metabolism, Vegetal Biology, Yeasts