The American journal of pathology, 2004-12, Vol.165 (6), p.2187-2196
2004
Details
- Autor(en) / Beteiligte
- Titel
- Novel Chemokine Responsiveness and Mobilization of Neutrophils during Sepsis
- Ist Teil von
- The American journal of pathology, 2004-12, Vol.165 (6), p.2187-2196
- Ort / Verlag
- Bethesda, MD: Elsevier Inc
- Erscheinungsjahr
- 2004
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Blood neutrophils (PMN) are usually unresponsive to CC chemokines such as monacyte chemotactic protein-1 and macrophage inflammatory protein-1α. In rodents, the lung buildup of PMN as determined by myeloperoxidase (MPO) activity after airway instillation of bacterial lipopolysaccharide (LPS) was independent of MCP-1 and MIP-1α. In striking contrast, during sepsis following cecal ligation and puncture (CLP), blood PMN demonstrated mRNA for CC chemokine receptors. Furthermore, PMN from CLP, but not from sham rodents, bound MCP-1 and MIP-1α and responded chemotactically in vitro to both MCP-1 and MIP-1α. In CCR2 −/− mice or WT mice treated in vivo with antibodies to either MCP-1 or MIP-1α, MPO activity was greatly attenuated in CLP animals. In CLP mice, increased serum IL-6 levels were found to be dependent on CCR2, MCP-1, and MIP-1α. When PMN from CLP rodents were incubated in vitro with either MCP-1 or MIP-1α, release of IL-6 was also shown. These findings suggest that sepsis fundamentally alters the trafficking of PMN into the lung in a manner that now engages functional responses to CC chemokines.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0002-9440eISSN: 1525-2191DOI: 10.1016/S0002-9440(10)63268-3Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1618724
- Format
- –
- Schlagworte
- Animals, Antibodies, Monoclonal - pharmacology, Biological and medical sciences, Cecum - injuries, Chemokine CCL2 - antagonists & inhibitors, Chemokine CCL2 - immunology, Chemokine CCL2 - metabolism, Chemokine CCL4, Interleukin-6 - metabolism, Investigative techniques, diagnostic techniques (general aspects), Lipopolysaccharides - pharmacology, Lung - cytology, Lung - immunology, Lung - metabolism, Macrophage Inflammatory Proteins - antagonists & inhibitors, Macrophage Inflammatory Proteins - immunology, Macrophage Inflammatory Proteins - metabolism, Male, Medical sciences, Mice, Mice, Inbred C57BL, Mice, Knockout, Neutrophils - immunology, Neutrophils - metabolism, Neutrophils - pathology, Original Research Paper, Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques, Peroxidase - metabolism, Receptors, CCR2, Receptors, Chemokine - genetics, Receptors, Chemokine - metabolism, Receptors, Chemokine - physiology, RNA, Messenger - genetics, RNA, Messenger - metabolism, Sepsis - genetics, Sepsis - immunology, Sepsis - metabolism