Details

Autor(en) / Beteiligte

• Martin, G.B. (Purdue University, West Lafayette, IN.)
• Frary, A
• Wu, T
• Brommonschendel, S
• Chunwongse, J
• Earle, E.D
• Tanksley, S.D

Titel

A member of the tomato Pto gene family confers sensitivity to fenthion resulting in rapid cell death

Ist Teil von

• The Plant cell, 1994-11, Vol.6 (11), p.1543-1552

Ort / Verlag

United States: American Society of Plant Physiologists

Erscheinungsjahr

1994

Quelle

MEDLINE

Beschreibungen/Notizen

• Leaves of tomato cultivars that contain the Pto bacterial resistance locus develop small necrotic lesions within 24 hr after exposure to fenthion an organophosphorous insecticide. Recently the Pto gene was isolated and shown to be a putative serine/threonine protein kinase. Pto is one member of a multigene family that is clustered within a 400-kb region on chromosome 5. Here we report that another member of this gene family termed Fen, is responsible for the sensitivity to fenthion. Fen was isolated by map-based cloning using closely linked DNA markers to identity a yeast artificial chromosome clone that spanned the Pto region. After transformation with the Fen gene under control of the cauliflower mosaic virus (CaMV) 35S promoter tomato plants that are normally insensitive to fenthion rapidly developed extensive necrotic lesions upon exposure to fenthion. Two related insecticides fensultothion and fenitrothion also elicited necrotic lesions specifically on Fen-transtormed plants. Transgenic tomato plants harboring integrated copies of the Pto gene under control of the CaMV 35S promoter displayed sensitivity to fenthion but to a lesser extent than did wild-type fenthion-sensitive plants. The Fen protein shares 80% identity (87% similarity) with Pto but does not conter resistance to Pseudomonas syringae pv tomato These results suggest that Pto and Fen participate in the same signal transduction pathway