Details

Autor(en) / Beteiligte

• Spalding, E.P. (Yale University, New Haven, CT)
• Goldsmith, M.H.M

Titel

Activation of K+ channels in the plasma membrane of Arabidopsis by ATP produced photosynthetically

Ist Teil von

• The Plant cell, 1993-04, Vol.5 (4), p.477-484

Ort / Verlag

Rockville, MD: American Society of Plant Physiologists

Erscheinungsjahr

1993

Quelle

Elektronische Zeitschriftenbibliothek (Open access)

Beschreibungen/Notizen

• Light activates a K+ channel and transiently depolarizes the plasma membrane of Arabidopsis mesophyll cells. Genetically or chemically impairing photosynthesis abolished this electrical response to light. These results indicate that illuminated chloroplasts produce a factor that activated K+ channels in the plasma membrane. By patch clamping at the single-channel level, we have obtained evidence that ATP is one such factor. Application of 0.2 to 2 mM ATP to the cytoplasmic side of excised patches of membrane reversibly activated the type of channel that was activated by light in cell-attached patches. In addition, an outward-rectifying K+ channel and an outward-rectifying nonselective cation channel were similarly activated by ATP, whereas a nonselective stretch-activated channel was unaffected by this treatment. This novel mechanism for controlling the permeability of the plasma membrane to K+ may be important to photosynthetic metabolism