Details

Autor(en) / Beteiligte

• Lügering, Andreas
• Floer, Martin
• Westphal, Sabine
• Maaser, Christian
• Spahn, Thomas W.
• Schmidt, M. Alexander
• Domschke, Wolfram
• Williams, Ifor R.
• Kucharzik, Torsten

Titel

Absence of CCR6 Inhibits CD4 + Regulatory T-Cell Development and M-Cell Formation inside Peyer's Patches

Ist Teil von

• The American journal of pathology, 2005-06, Vol.166 (6), p.1647-1654

Ort / Verlag

Bethesda, MD: Elsevier Inc

Erscheinungsjahr

2005

Link zum Volltext

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• The chemokine Mip3α is specifically expressed by the follicle-associated epithelia (FAE) covering intestinal Peyer's patches (PPs) and is the only known chemokine ligand for the chemokine receptor CCR6. Although CCR6-deficient mice are known to have a perturbed intestinal immune system, little is known about the specific impact of this interaction for Peyer's patch formation. To elucidate the effect of Mip3α on PP lymphocyte development, we used a CCR6/enhanced green fluorescent protein (EGFP) knock-in mouse model and analyzed lymphocyte development by immunohistochemistry and flow cytometry. PPs of CCR6 −/− mice were significantly size-reduced with a proportional loss of B cells and T cells, whereas T-cell subsets were disturbed with a decreased CD4/CD8 ratio paralleled with a loss of regulatory CD4 + CD45Rb low T cells. The analysis of cytokine production by CCR6-expressing cells could demonstrate that CCR6 is involved in the regulation of cytokine secretion such as interleukin-12 by dendritic cells. Quantification of UEA-1 + cells inside the FAE showed reduced M-cell numbers in CCR6-deficient mice. These results suggest that the interaction of CCR6 with its ligand Mip3α is important for immune responses generated inside the PPs, particularly for the generation of regulatory CD4 + T cells residing inside PPs and for the formation of M cells.