Details

Autor(en) / Beteiligte

• Childs, K.L. (Texas AandM University, College Station.)
• Miller, F.R
• Cordonnier-Pratt, M.M
• Pratt, L.H
• Morgan, P.W
• Mullet, J.E

Titel

The sorghum photoperiod sensitivity gene, Ma3, encodes a phytochrome B

Ist Teil von

• Plant physiology (Bethesda), 1997-02, Vol.113 (2), p.611-619

Ort / Verlag

Rockville, MD: American Society of Plant Physiologists

Erscheinungsjahr

1997

Quelle

MEDLINE

Beschreibungen/Notizen

• The Ma3 gene is one of six genes that regulate the photoperiodic sensitivity of flowering in sorghum (Sorghum bicolor [L.] Moench). The ma3R mutation of this gene causes a phenotype that is similar to plants that are known to lack phytochrome B, and ma3R sorghum lacks a 123-kD phytochrome that predominates in light-grown plants and that is present in non-ma3R plants. A population segregating for Ma3 and ma3R was created and used to identify two randomly amplified polymorphic DNA markers linked to Ma3. These two markers were cloned and mapped in a recombinant inbred population as restriction fragment length polymorphisms. cDNA clones of PHYA and PHYC were cloned and sequenced from a cDNA library prepared from green sorghum leaves. Using a genome-walking technique, a 7941-bp partial sequence of PHYB was determined from genomic DNA from ma3R sorghum. PHYA, PHYB, and PHYC all mapped to the same linkage group. The Ma3-linked markers mapped with PHYB more than 121 centimorgans from PHYA and PHYC. A frameshift mutation resulting in a premature stop codon was found in the PHYB sequence from ma3R sorghum. Therefore, we conclude that the Ma3 locus in sorghum is a PHYB gene that encodes a 123-kD phytochrome