British journal of pharmacology, 2004-04, Vol.141 (7), p.1091-1097
2004
Details
- Autor(en) / Beteiligte
- Titel
- Differential regulation of prostaglandin E biosynthesis by interferon‐γ in colonic epithelial cells
- Ist Teil von
- British journal of pharmacology, 2004-04, Vol.141 (7), p.1091-1097
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 2004
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Cyclooxygenase (COX)‐2 expression and activity in response to pro‐inflammatory cytokines TNFα and IFNγ was evaluated in the colonic epithelial cell line HT29 and the airway epithelial cell line A549. TNFα induced concentration‐ and time‐dependent upregulation of COX‐2 mRNA, protein and prostaglandin (PG)E2 synthesis. Co‐stimulation of TNFα with IFNγ resulted in reduced COX‐2 mRNA and protein expression. IFNγ had no effect on the stability of TNFα‐induced COX‐2 mRNA. TNFα‐induced PGE2 biosynthesis was significantly enhanced by the simultaneous addition of IFNγ and was COX‐2 dependent. The combination of IFNγ and TNFα induced the microsomal prostaglandin E synthase (mPGES), comensurate with the enhanced PGE2 synthesis. These results suggest that, in terms of PGE2 biosynthesis, IFNγ plays a negative regulatory role at the level of COX‐2 expression and a positive regulatory role at the level of mPGES expression. This may have important implications for the clinical use of IFNγ in inflammatory diseases. British Journal of Pharmacology (2004) 141, 1091–1097. doi:10.1038/sj.bjp.0705719
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0007-1188eISSN: 1476-5381DOI: 10.1038/sj.bjp.0705719Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1574887
- Format
- –
- Schlagworte
- Animals, Biological and medical sciences, Caco-2 Cells, colon, Colon - drug effects, Colon - metabolism, Colon - pathology, cyclooxygenase, Cyclooxygenase Inhibitors - metabolism, cytokine, Dactinomycin - pharmacology, Dose-Response Relationship, Drug, Drug Therapy, Combination, Epithelial Cells - drug effects, Epithelial Cells - metabolism, Epithelial Cells - pathology, Evaluation Studies as Topic, HT29 Cells, Humans, Inflammation, Interferon-alpha - metabolism, Interferon-alpha - pharmacology, Interferon-gamma - antagonists & inhibitors, Interferon-gamma - metabolism, Interferon-gamma - pharmacology, Intramolecular Oxidoreductases - genetics, Intramolecular Oxidoreductases - metabolism, Medical sciences, Mice, Microsomes - enzymology, Pharmacology. Drug treatments, prostaglandin, Prostaglandin-E Synthases, Prostaglandins E - biosynthesis, RNA, Messenger - genetics, RNA, Messenger - metabolism, synthase, Tumor Necrosis Factor-alpha - drug effects, Tumor Necrosis Factor-alpha - metabolism, Tumor Necrosis Factor-alpha - pharmacology, Up-Regulation