Details

Autor(en) / Beteiligte

• Pedrazzini, Emanuela
• Giovinazzo, Giovanna
• Bielli, Anna
• de Virgilio, Maddalena
• Frigerio, Lorenzo
• Pesca, Michela
• Faoro, Franco
• Bollini, Roberto
• Ceriotti, Aldo
• Vitale, Alessandro

Titel

Protein Quality Control along the Route to the Plant Vacuole

Ist Teil von

• The Plant cell, 1997-10, Vol.9 (10), p.1869-1880

Ort / Verlag

England: American Society of Plant Physiologists

Erscheinungsjahr

1997

Quelle

MEDLINE

Beschreibungen/Notizen

• To acquire information on the relationships between structural maturation of proteins in the endoplasmic reticulum (ER) and their transport along the secretory pathway, we have analyzed the destiny of an assembly-defective form of the trimeric vacuolar storage glycoprotein phaseolin. In leaves of transgenic tobacco, where assembly-competent phaseolin is correctly targeted to the vacuole, defective phaseolin remains located in the ER or a closely related compartment where it represents a major ligand of the chaperone BiP. Defective phaseolin maintained susceptibility to endoglycosidase H and was slowly degraded by a process that is not inhibited by heat shock or brefeldin A, indicating that degradation does not involve transport along the secretory pathway. These results provide evidence for the presence of a quality control mechanism in the ER of plant cells that avoids intracellular trafficking of severely defective proteins and eventually leads to their degradation.