Proceedings of the National Academy of Sciences - PNAS, 2006-02, Vol.103 (6), p.1988-1993
2006
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- Autor(en) / Beteiligte
- Titel
- Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 2006-02, Vol.103 (6), p.1988-1993
- Ort / Verlag
- United States: National Academy of Sciences
- Erscheinungsjahr
- 2006
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- bZIP-type transcription factors AREBs/ABFs bind an abscisic acid (ABA)-responsive cis-acting element named ABRE and transactivate downstream gene expression in Arabidopsis. Because AREB1 overexpression could not induce downstream gene expression, activation of AREB1 requires ABA-dependent posttranscriptional modification. We confirmed that ABA activated 42-kDa kinase activity, which, in turn, phosphorylated Ser/Thr residues of R-X-X-S/T sites in the conserved regions of AREB1. Amino acid substitutions of R-X-X-S/T sites to Ala suppressed transactivation activity, and multiple substitution of these sites resulted in almost complete suppression of transactivation activity in transient assays. In contrast, substitution of the Ser/Thr residues to Asp resulted in high transactivation activity without exogenous ABA application. A phosphorylated, transcriptionally active form was achieved by substitution of Ser/Thr in all conserved R-X-X-S/T sites to Asp. Transgenic plants overexpressing the phosphorylated active form of AREB1 expressed many ABA-inducible genes, such as RD29B, without ABA treatment. These results indicate that the ABA-dependent multisite phosphorylation of AREB1 regulates its own activation in plants.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.0505667103Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1413621
- Format
- –
- Schlagworte
- abscisic acid, Abscisic Acid - pharmacology, abscisic acid-responsive element, Amino Acid Sequence, Amino Acid Substitution, Arabidopsis, Arabidopsis - chemistry, Arabidopsis - drug effects, Arabidopsis - growth & development, Arabidopsis - metabolism, Arabidopsis Proteins - chemistry, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, Arabidopsis thaliana, Basic-Leucine Zipper Transcription Factors - chemistry, Basic-Leucine Zipper Transcription Factors - genetics, Basic-Leucine Zipper Transcription Factors - metabolism, binding capacity, Binding sites, Biological Sciences, Botany, Conserved Sequence, enzyme activity, Flowers & plants, Gene expression, Gene Expression Regulation, Plant - drug effects, gene overexpression, Genes, Molecular Sequence Data, Mutation - genetics, Oligonucleotide Array Sequence Analysis, Peptide Fragments - chemistry, Peptide Fragments - genetics, Peptide Fragments - metabolism, Phosphorylation, Phosphorylation - drug effects, Plant cells, Plants, promoter regions, protein phosphorylation, Proteins, Protoplasts, RNA, Messenger - genetics, Sequence Alignment, signal transduction, Trans-Activators - chemistry, Trans-Activators - genetics, Trans-Activators - metabolism, Transactivation, transcription (genetics), transcription factors, transcriptional activation, Transgenic plants