Details

Autor(en) / Beteiligte

• Myers, J.K.
• Pace, C.N.

Titel

Hydrogen bonding stabilizes globular proteins

Ist Teil von

• Biophysical journal, 1996-10, Vol.71 (4), p.2033-2039

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

1996

Quelle

MEDLINE

Beschreibungen/Notizen

• It is clear that intramolecular hydrogen bonds are essential to the structure and stability of globular proteins. It is not clear, however, whether they make a net favorable contribution to this stability. Experimental and theoretical studies are at odds over this important question. Measurements of the change in conformational stability, delta (delta G), for the mutation of a hydrogen bonded residue to one incapable of hydrogen bonding suggest a stabilization of 1.0 kcal/mol per hydrogen bond. If the delta (delta G) values are corrected for differences in side-chain hydrophobicity and conformational entropy, then the estimated stabilization becomes 2.2 kcal/mol per hydrogen bond. These and other experimental studies discussed here are consistent and compelling: hydrogen bonding stabilizes globular proteins.