The EMBO journal, 1997-04, Vol.16 (8), p.1842-1849
1997
Details
- Autor(en) / Beteiligte
- Titel
- Sequential action of two hsp70 complexes during protein import into mitochondria
- Ist Teil von
- The EMBO journal, 1997-04, Vol.16 (8), p.1842-1849
- Ort / Verlag
- Chichester, UK: John Wiley & Sons, Ltd
- Erscheinungsjahr
- 1997
- Link zum Volltext
- Quelle
- Wiley Online Library - AutoHoldings Journals
- Beschreibungen/Notizen
- The mitochondrial chaperone mhsp70 mediates protein transport across the inner membrane and protein folding in the matrix. These two reactions are effected by two different mhsp70 complexes. The ADP conformation of mhsp70 favors formation of a complex on the inner membrane; this 'import complex' contains mhsp70, its membrane anchor Tim44 and the nucleotide exchange factor mGrpE. The ATP conformation of mhsp70 favors formation of a complex in the matrix; this 'folding complex' contains mhsp70, the mitochondrial DnaJ homolog Mdj1 and mGrpE. A precursor protein entering the matrix interacts first with the import complex and then with the folding complex. A chaperone can thus function as part of two different complexes within the same organelle.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0261-4189, 1460-2075eISSN: 1460-2075DOI: 10.1093/emboj/16.8.1842Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1169787
- Format
- –
- Schlagworte
- adenosine diphosphate, Adenosine Diphosphate - metabolism, Adenosine Triphosphatases - metabolism, adenosine triphosphate, Adenosine Triphosphate - metabolism, adenosinetriphosphatase, binding, binding proteins, Carrier Proteins - metabolism, cell membranes, chaperone, enzyme activity, Folic Acid Antagonists - pharmacology, Fungal Proteins - chemistry, Fungal Proteins - genetics, Fungal Proteins - metabolism, heat shock proteins, Heat-Shock Proteins - chemistry, Heat-Shock Proteins - genetics, Heat-Shock Proteins - metabolism, Histidine, HSP40 Heat-Shock Proteins, HSP70 Heat-Shock Proteins - chemistry, HSP70 Heat-Shock Proteins - genetics, HSP70 Heat-Shock Proteins - metabolism, Intracellular Membranes, L-Lactate Dehydrogenase (Cytochrome), L-Lactate Dehydrogenase - genetics, L-Lactate Dehydrogenase - metabolism, Membrane Proteins - chemistry, Membrane Proteins - genetics, Membrane Proteins - metabolism, Membrane Transport Proteins, Methotrexate - pharmacology, mitochondria, Mitochondria - metabolism, mitochondrial inner membranes, Mitochondrial Membrane Transport Proteins, Molecular Chaperones, molecular conformation, Peptides, Protein Binding, Protein Folding, Protein Precursors - metabolism, protein translocation, protein transport, Saccharomyces cerevisiae, Saccharomyces cerevisiae - metabolism, Saccharomyces cerevisiae Proteins, Tetrahydrofolate Dehydrogenase - genetics, Tetrahydrofolate Dehydrogenase - metabolism, yeast