Details

Autor(en) / Beteiligte

• Garrido-del Solo, C
• García-Cánovas, F
• Havsteen, B H
• Valero, E
• Varón, R

Titel

Kinetics of an enzyme reaction in which both the enzyme-substrate complex and the product are unstable or only the product is unstable

Ist Teil von

• Biochemical journal, 1994-10, Vol.303 ( Pt 2) (2), p.435-440

Ort / Verlag

England

Erscheinungsjahr

1994

Quelle

MEDLINE

Beschreibungen/Notizen

• A kinetic analysis of the Michaelis-Menten mechanism has been made for the case in which both the enzyme-substrate complex and the product are unstable or only the product is unstable, either spontaneously or as the result of the addition of a reagent. This analysis allows the derivation of equations which under conditions of limiting enzyme concentration relate the concentration of all of the species to the time. A kinetic data analysis is suggested, which leads to the evaluation of the kinetic parameters involved in the reaction. The analysis is based on the equation which describes the formation of products with time and one's experimental progress curves. We demonstrate the method numerically by computer simulation of the reaction with added experimental errors and experimentally by the use of data from the kinetic study of the action of tyrosinase on dopamine.