Journal of bacteriology, 2000-10, Vol.182 (19), p.5624-5627
2000
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- Autor(en) / Beteiligte
- Titel
- Purification and characterization of glpX-encoded fructose 1, 6-bisphosphatase, a new enzyme of the glycerol 3-phosphate regulon of Escherichia coli
- Ist Teil von
- Journal of bacteriology, 2000-10, Vol.182 (19), p.5624-5627
- Ort / Verlag
- United States: American Society for Microbiology
- Erscheinungsjahr
- 2000
- Quelle
- Electronic Journals Library
- Beschreibungen/Notizen
- In Escherichia coli, gene products of the glp regulon mediate utilization of glycerol and sn-glycerol 3-phosphate. The glpFKX operon encodes glycerol diffusion facilitator, glycerol kinase, and as shown here, a fructose 1,6-bisphosphatase that is distinct from the previously described fbp-encoded enzyme. The purified enzyme was dimeric, dependent on Mn(2+) for activity, and exhibited an apparent K(m) of 35 microM for fructose 1,6-bisphosphate. The enzyme was inhibited by ADP and phosphate and activated by phosphoenolpyruvate.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9193eISSN: 1098-5530DOI: 10.1128/JB.182.19.5624-5627.2000Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_111013
- Format
- –
- Schlagworte
- ADP, Bacteria, Bacterial Proteins - genetics, Bacterial Proteins - isolation & purification, Bacterial Proteins - metabolism, Bacterial Proteins - physiology, Bacteriology, Biochemistry, Catalysis, Cloning, Molecular, Enzymes, Enzymes and Proteins, Escherichia coli, Escherichia coli - enzymology, Escherichia coli - genetics, Escherichia coli Proteins, fructose 1,6-bisphosphatase, Fructose-Bisphosphatase - genetics, Fructose-Bisphosphatase - isolation & purification, Fructose-Bisphosphatase - metabolism, Fructose-Bisphosphatase - physiology, Gene Expression, Genes, glpF gene, glpK gene, glpX gene, glycerol 3-phosphate, Glycerophosphates - metabolism, Molecular Weight, phosphoenolpyruvate, Regulon, Substrate Specificity