Details

Autor(en) / Beteiligte

• Xu, Guogang
• Quan, Songhua
• Schell, Joseph
• Gao, Yucheng
• Varmazyad, Mahboubeh
• Sreenivas, Prethish
• Cruz, Diego
• Jiang, Haiyan
• Pan, Meixia
• Han, Xianlin
• Palavicini, Juan Pablo
• Zhao, Peng
• Sun, Xiaoli
• Marchant, Erik D
• Rasmussen, Blake B
• Li, Guannan
• Katsumura, Sakie
• Morita, Masahiro
• Munkácsy, Erin
• Horikoshi, Nobuo
• Chocron, E Sandra
• Gius, David

Titel

Mitochondrial ACSS1-K635 acetylation knock-in mice exhibit altered metabolism, cell senescence, and nonalcoholic fatty liver disease

Ist Teil von

• Science advances, 2024-05, Vol.10 (20), p.eadj5942

Ort / Verlag

United States: American Association for the Advancement of Science

Erscheinungsjahr

2024

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Acetyl-CoA synthetase short-chain family member 1 (ACSS1) uses acetate to generate mitochondrial acetyl-CoA and is regulated by deacetylation by sirtuin 3. We generated an ACSS1-acetylation (Ac) mimic mouse, where lysine-635 was mutated to glutamine (K635Q). Male mice were smaller with higher metabolic rate and blood acetate and decreased liver/serum ATP and lactate levels. After a 48-hour fast, mice presented hypothermia and liver aberrations, including enlargement, discoloration, lipid droplet accumulation, and microsteatosis, consistent with nonalcoholic fatty liver disease (NAFLD). RNA sequencing analysis suggested dysregulation of fatty acid metabolism, cellular senescence, and hepatic steatosis networks, consistent with NAFLD. Fasted mouse livers showed increased fatty acid synthase (FASN) and stearoyl-CoA desaturase 1 (SCD1), both associated with NAFLD, and increased carbohydrate response element-binding protein binding to and enhancer regions. Last, liver lipidomics showed elevated ceramide, lysophosphatidylethanolamine, and lysophosphatidylcholine, all associated with NAFLD. Thus, we propose that ACSS1-K635-Ac dysregulation leads to aberrant lipid metabolism, cellular senescence, and NAFLD.