Details

Autor(en) / Beteiligte

• Luo, Rong-Guang
• Wu, Yi-Fan
• Lu, Hai-Wen
• Weng, Dong
• Xu, Jia-Yan
• Wang, Le-Le
• Zhang, Li-Sha
• Zhao, Cai-Qi
• Li, Jian-Xiong
• Yu, Yong
• Jia, Xin-Ming
• Xu, Jin-Fu

Titel

Th2-skewed peripheral T-helper cells drive B-cells in allergic bronchopulmonary aspergillosis

Ist Teil von

• The European respiratory journal, 2024-05, Vol.63 (5), p.2400386

Ort / Verlag

England: European Respiratory Society

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Patients with allergic bronchopulmonary aspergillosis (ABPA) suffer from repeated exacerbations. The involvement of T-cell subsets remains unclear. We enrolled ABPA patients, asthma patients and healthy controls. T-helper type 1 (Th1), 2 (Th2) and 17 (Th17) cells, regulatory T-cells (Treg) and interleukin (IL)-21 CD4 T-cells in total or sorted subsets of peripheral blood mononuclear cells and ABPA bronchoalveolar lavage fluid (BALF) were analysed using flow cytometry. RNA sequencing of subsets of CD4 T-cells was done in exacerbated ABPA patients and healthy controls. Antibodies of T-/B-cell co-cultures were measured. ABPA patients had increased Th2 cells, similar numbers of Treg cells and decreased circulating Th1 and Th17 cells. IL-5 IL-13 IL-21 CD4 T-cells were rarely detected in healthy controls, but significantly elevated in the blood of ABPA patients, especially the exacerbated ones. We found that IL-5 IL-13 IL-21 CD4 T-cells were mainly peripheral T-helper (Tph) cells (PD-1 CXCR5 ), which also presented in the BALF of ABPA patients. The proportions of circulating Tph cells were similar among ABPA patients, asthma patients and healthy controls, while IL-5 IL-13 IL-21 Tph cells significantly increased in ABPA patients. Transcriptome data showed that Tph cells of ABPA patients were Th2-skewed and exhibited signatures of follicular T-helper cells. When co-cultured , Tph cells of ABPA patients induced the differentiation of autologous B-cells into plasmablasts and significantly enhanced the production of IgE. We identified a distinctly elevated population of circulating Th2-skewed Tph cells that induced the production of IgE in ABPA patients. It may be a biomarker and therapeutic target for ABPA.