Details

Autor(en) / Beteiligte

• Shafiq, Tiasha A
• Yu, Juntao
• Feng, Wenzhi
• Zhang, Yizhe
• Zhou, Haining
• Paulo, Joao A
• Gygi, Steven P
• Moazed, Danesh

Titel

Genomic context- and H2AK119 ubiquitination-dependent inheritance of human Polycomb silencing

Ist Teil von

• Science advances, 2024-05, Vol.10 (19), p.eadl4529-eadl4529

Ort / Verlag

United States: American Association for the Advancement of Science

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Polycomb repressive complexes 1 and 2 (PRC1 and 2) are required for heritable repression of developmental genes. The cis- and trans-acting factors that contribute to epigenetic inheritance of mammalian Polycomb repression are not fully understood. Here, we show that, in human cells, ectopically induced Polycomb silencing at initially active developmental genes, but not near ubiquitously expressed housekeeping genes, is inherited for many cell divisions. Unexpectedly, silencing is heritable in cells with mutations in the H3K27me3 binding pocket of the Embryonic Ectoderm Development (EED) subunit of PRC2, which are known to disrupt H3K27me3 recognition and lead to loss of H3K27me3. This mode of inheritance is less stable and requires intact PRC2 and recognition of H2AK119ub1 by PRC1. Our findings suggest that maintenance of Polycomb silencing is sensitive to local genomic context and can be mediated by PRC1-dependent H2AK119ub1 and PRC2 independently of H3K27me3 recognition.