Details

Autor(en) / Beteiligte

• Xing, Fanfan
• Deng, Chaowen
• Luo, Zhendong
• Zou, Shan
• Liu, Min
• Ye, Haiyan
• Sun, Linlin
• Tsang, Chi-Ching
• Lo, Simon K. F.
• Lau, Susanna K. P.
• Woo, Patrick C. Y.

Titel

Rapid Diagnosis of Pneumocystis jirovecii Pneumonia and Respiratory Tract Colonization by Next-Generation Sequencing

Ist Teil von

• Mycopathologia (1975), 2024-06, Vol.189 (3), p.38-38

Ort / Verlag

Dordrecht: Springer Netherlands

Erscheinungsjahr

2024

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• Objectives To describe the epidemiology of Pneumocystis jirovecii pneumonia and colonization diagnosed by next-generation sequencing (NGS) and explore the usefulness of the number of P. jirovecii sequence reads for the diagnosis of P. jirovecii pneumonia. Methods We examined the NGS results for P. jirovecii in respiratory samples collected from patients and analysed their clinical, radiological and microbiological characteristics. Results Among 285 respiratory samples collected over a 12-month period (January to December 2022), P. jirovecii sequences were detected in 56 samples from 53 patients. Fifty (94.3%) of the 53 patients were HIV-negative. Following our case definitions, 37 (69.8%) and 16 (30.2%) of the 53 patients had P. jirovecii infection and colonization respectively. P. jirovecii infection was associated with presence of underlying disease with immunosuppression (94.6% vs 18.8%, P  < 0.05), positive serum 1,3-β-D-glucan (41.2% vs 0%, P  < 0.01) and higher number of P. jirovecii sequence reads ( P  < 0.005). In contrast, P. jirovecii colonization was associated with the male sex (93.8% vs 54.1%, P  < 0.01), another definitive infectious disease diagnosis of the respiratory tract (43.8% vs 2.7%, P  < 0.001) and higher survival (100% vs 67.6%, P  < 0.01). Although P. jirovecii pneumonia was associated with higher number of P. jirovecii reads in respiratory samples, only a sensitivity of 82.14% and a specificity of 68.75% could be achieved. Conclusion Detection of P. jirovecii sequences in respiratory samples has to be interpreted discreetly. A combination of clinical, radiological and laboratory findings is still the most crucial in determining whether a particular case is genuine P. jirovecii pneumonia.