Details

Autor(en) / Beteiligte

• Garcia-Medina, J Sebastian
• Sienkiewicz, Karolina
• Narayanan, S Anand
• Overbey, Eliah G
• Grigorev, Kirill
• Ryon, Krista A
• Burke, Marissa
• Proszynski, Jacqueline
• Tierney, Braden
• Schmidt, Caleb M
• Mencia-Trinchant, Nuria
• Klotz, Remi
• Ortiz, Veronica
• Foox, Jonathan
• Chin, Christopher
• Najjar, Deena
• Matei, Irina
• Chan, Irenaeus
• Cruchaga, Carlos
• Kleinman, Ashley
• Kim, JangKeun
• Lucaci, Alexander
• Loy, Conor
• Mzava, Omary
• De Vlaminck, Iwijn
• Singaraju, Anvita
• Taylor, Lynn E
• Schmidt, Julian C
• Schmidt, Michael A
• Blease, Kelly
• Moreno, Juan
• Boddicker, Andrew
• Zhao, Junhua
• Lajoie, Bryan
• Altomare, Andrew
• Kruglyak, Semyon
• Levy, Shawn
• Yu, Min
• Hassane, Duane C
• Bailey, Susan M
• Bolton, Kelly
• Mateus, Jaime
• Mason, Christopher E

Titel

Genome and clonal hematopoiesis stability contrasts with immune, cfDNA, mitochondrial, and telomere length changes during short duration spaceflight

Ist Teil von

• Precision clinical medicine, 2024-03, Vol.7 (1), p.pbae007-pbae007

Ort / Verlag

England: Oxford University Press

Erscheinungsjahr

2024

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The Inspiration4 (I4) mission, the first all-civilian orbital flight mission, investigated the physiological effects of short-duration spaceflight through a multi-omic approach. Despite advances, there remains much to learn about human adaptation to spaceflight's unique challenges, including microgravity, immune system perturbations, and radiation exposure. To provide a detailed genetics analysis of the mission, we collected dried blood spots pre-, during, and post-flight for DNA extraction. Telomere length was measured by quantitative PCR, while whole genome and cfDNA sequencing provided insight into genomic stability and immune adaptations. A robust bioinformatic pipeline was used for data analysis, including variant calling to assess mutational burden. Telomere elongation occurred during spaceflight and shortened after return to Earth. Cell-free DNA analysis revealed increased immune cell signatures post-flight. No significant clonal hematopoiesis of indeterminate potential (CHIP) or whole-genome instability was observed. The long-term gene expression changes across immune cells suggested cellular adaptations to the space environment persisting months post-flight. Our findings provide valuable insights into the physiological consequences of short-duration spaceflight, with telomere dynamics and immune cell gene expression adapting to spaceflight and persisting after return to Earth. CHIP sequencing data will serve as a reference point for studying the early development of CHIP in astronauts, an understudied phenomenon as previous studies have focused on career astronauts. This study will serve as a reference point for future commercial and non-commercial spaceflight, low Earth orbit (LEO) missions, and deep-space exploration.