Communications biology, 2024-03, Vol.7 (1), p.345-345
2024
Details
- Autor(en) / Beteiligte
- Titel
- AKAP150-anchored PKA regulates synaptic transmission and plasticity, neuronal excitability and CRF neuromodulation in the mouse lateral habenula
- Ist Teil von
- Communications biology, 2024-03, Vol.7 (1), p.345-345
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2024
- Link zum Volltext
- Quelle
- EZB Free E-Journals
- Beschreibungen/Notizen
- The scaffolding A-kinase anchoring protein 150 (AKAP150) is critically involved in kinase and phosphatase regulation of synaptic transmission/plasticity, and neuronal excitability. Emerging evidence also suggests that AKAP150 signaling may play a key role in brain's processing of rewarding/aversive experiences, however its role in the lateral habenula (LHb, as an important brain reward circuitry) is completely unknown. Using whole cell patch clamp recordings in LHb of male wildtype and ΔPKA knockin mice (with deficiency in AKAP-anchoring of PKA), here we show that the genetic disruption of PKA anchoring to AKAP150 significantly reduces AMPA receptor-mediated glutamatergic transmission and prevents the induction of presynaptic endocannabinoid-mediated long-term depression in LHb neurons. Moreover, ΔPKA mutation potentiates GABA receptor-mediated inhibitory transmission while increasing LHb intrinsic excitability through suppression of medium afterhyperpolarizations. ΔPKA mutation-induced suppression of medium afterhyperpolarizations also blunts the synaptic and neuroexcitatory actions of the stress neuromodulator, corticotropin releasing factor (CRF), in mouse LHb. Altogether, our data suggest that AKAP150 complex signaling plays a critical role in regulation of AMPA and GABA receptor synaptic strength, glutamatergic plasticity and CRF neuromodulation possibly through AMPA receptor and potassium channel trafficking and endocannabinoid signaling within the LHb.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 2399-3642DOI: 10.1038/s42003-024-06041-8Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10954712
- Format
- –
- Schlagworte
- A Kinase Anchor Proteins - genetics, A Kinase Anchor Proteins - metabolism, A kinase-anchoring protein, Animals, Corticotropin-releasing hormone, Corticotropin-Releasing Hormone - metabolism, Endocannabinoids, Excitability, Glutamatergic transmission, Habenula, Habenula - metabolism, Kinases, Long-term depression, Luteinizing hormone, Male, Mice, Mutation, Neuromodulation, Neuronal Plasticity - physiology, Neurons - physiology, Proteins, Receptors, AMPA - genetics, Receptors, AMPA - metabolism, Receptors, GABA-A - metabolism, Synaptic plasticity, Synaptic strength, Synaptic transmission, Synaptic Transmission - physiology, α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid, α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors, γ-Aminobutyric acid A receptors