Details

Autor(en) / Beteiligte

• Vallina, Néstor Sampedro
• McRae, Ewan K S
• Geary, Cody
• Andersen, Ebbe S

Titel

An RNA origami robot that traps and releases a fluorescent aptamer

Ist Teil von

• Science advances, 2024-03, Vol.10 (12), p.eadk1250-eadk1250

Ort / Verlag

United States: American Association for the Advancement of Science

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• RNA nanotechnology aims to use RNA as a programmable material to create self-assembling nanodevices for application in medicine and synthetic biology. The main challenge is to develop advanced RNA robotic devices that both sense, compute, and actuate to obtain enhanced control over molecular processes. Here, we use the RNA origami method to prototype an RNA robotic device, named the "Traptamer," that mechanically traps the fluorescent aptamer, iSpinach. The Traptamer is shown to sense two RNA key strands, acts as a Boolean AND gate, and reversibly controls the fluorescence of the iSpinach aptamer. Cryo-electron microscopy of the closed Traptamer structure at 5.45-angstrom resolution reveals the mechanical mode of distortion of the iSpinach motif. Our study suggests a general approach to distorting RNA motifs and a path forward to build sophisticated RNA machines that through sensing, computing, and actuation modules can be used to precisely control RNA functionalities in cellular systems.