Details

Autor(en) / Beteiligte

• Duan, Yuange
• Ma, Ling
• Liu, Jiyao
• Liu, Xinzhi
• Song, Fan
• Tian, Li
• Cai, Wanzhi
• Li, Hu

Titel

The first A-to-I RNA editome of hemipteran species Coridius chinensis reveals overrepresented recoding and prevalent intron editing in early-diverging insects

Ist Teil von

• Cellular and molecular life sciences : CMLS, 2024-12, Vol.81 (1), p.136-136

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2024

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• Background Metazoan adenosine-to-inosine (A-to-I) RNA editing resembles A-to-G mutation and increases proteomic diversity in a temporal-spatial manner, allowing organisms adapting to changeable environment. The RNA editomes in many major animal clades remain unexplored, hampering the understanding on the evolution and adaptation of this essential post-transcriptional modification. Methods We assembled the chromosome-level genome of Coridius chinensis belonging to Hemiptera, the fifth largest insect order where RNA editing has not been studied yet. We generated ten head RNA-Seq libraries with DNA-Seq from the matched individuals. Results We identified thousands of high-confidence RNA editing sites in C. chinensis . Overrepresentation of nonsynonymous editing was observed, but conserved recoding across different orders was very rare. Under cold stress, the global editing efficiency was down-regulated and the general transcriptional processes were shut down. Nevertheless, we found an interesting site with “conserved editing but non-conserved recoding” in potassium channel Shab which was significantly up-regulated in cold, serving as a candidate functional site in response to temperature stress. Conclusions RNA editing in C. chinensis largely recodes the proteome. The first RNA editome in Hemiptera indicates independent origin of beneficial recoding during insect evolution, which advances our understanding on the evolution, conservation, and adaptation of RNA editing.