Details

Autor(en) / Beteiligte

• Yu, Ching Tzu
• Kandoi, Sangeetha
• Periasamy, Ramesh
• Reddy, L. Vinod K.
• Follett, Hannah M.
• Summerfelt, Phyllis
• Martinez, Cassandra
• Guillaume, Chloe
• Bowie, Owen
• Connor, Thomas B.
• Lipinski, Daniel M.
• Allen, Kenneth P.
• Merriman, Dana K.
• Carroll, Joseph
• Lamba, Deepak A.

Titel

Human iPSC-derived photoreceptor transplantation in the cone dominant 13-lined ground squirrel

Ist Teil von

• Stem cell reports, 2024-03, Vol.19 (3), p.331-342

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2024

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Several retinal degenerations affect the human central retina, which is primarily comprised of cones and is essential for high acuity and color vision. Transplanting cone photoreceptors is a promising strategy to replace degenerated cones in this region. Although this approach has been investigated in a handful of animal models, commonly used rodent models lack a cone-rich region and larger models can be expensive and inaccessible, impeding the translation of therapies. Here, we transplanted dissociated GFP-expressing photoreceptors from retinal organoids differentiated from human induced pluripotent stem cells into the subretinal space of damaged and undamaged cone-dominant 13-lined ground squirrel eyes. Transplanted cell survival was documented via noninvasive high-resolution imaging and immunohistochemistry to confirm the presence of human donor photoreceptors for up to 4 months posttransplantation. These results demonstrate the utility of a cone-dominant rodent model for advancing the clinical translation of cell replacement therapies. •Successful integration of hiPSC photoreceptors was observed in the 13-LGS retina•Noninvasive longitudinal imaging traced transplanted cells for up to 4 months•Maturation structures resembling inner segments was visualized using AOSLO imaging•Degree of integration was related to type of damage in the induced 13-LGS models Yu and colleagues showed that human iPSC-derived photoreceptors generated from 3D-retinal organoids could survive up to 4 months following transplantation in the cone-dominant 13-lined ground squirrel retina. The degree of integration and intraretinal localization of the transplanted cells appeared to depend on the degree of retinal damage.