Details

Autor(en) / Beteiligte

• Huang, Chengcheng
• Seino, Junichi
• Honda, Akinobu
• Fujihira, Haruhiko
• Wu, Di
• Okahara, Kyohei
• Kitazume, Shinobu
• Nakaya, Shuichi
• Kitajima, Ken
• Sato, Chihiro
• Suzuki, Tadashi

Titel

Rat hepatocytes secrete free oligosaccharides

Ist Teil von

• The Journal of biological chemistry, 2024-03, Vol.300 (3), p.105712-105712, Article 105712

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2024

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• We recently established a method for the isolation of serum-free oligosaccharides, and characterized various features of their structures. However, the precise mechanism for how these glycans are formed still remains unclarified. To further investigate the mechanism responsible for these serum glycans, here, we utilized rat primary hepatocytes to examine whether they are able to secrete free glycans. Our findings indicated that a diverse array of free oligosaccharides such as sialyl/neutral free N-glycans (FNGs), as well as sialyl lactose/LacNAc-type glycans, were secreted into the culture medium by primary hepatocytes. The structural features of these free glycans in the medium were similar to those isolated from the sera of the same rat. Further evidence suggested that an oligosaccharyltransferase is involved in the release of the serum-free N-glycans. Our results indicate that the liver is indeed secreting various types of free glycans directly into the serum.