Details

Autor(en) / Beteiligte

• Gargis, Amy S
• Karlsson, Maria
• Paulick, Ashley L
• Anderson, Karen F
• Adamczyk, Michelle
• Vlachos, Nicholas
• Kent, Alyssa G
• McAllister, Gillian
• McKay, Susannah L
• Halpin, Alison L
• Albrecht, Valerie
• Campbell, Davina
• Korhonen, Lauren C
• Elkins, Christopher A
• Rasheed, J Kamile
• Guh, Alice Y
• McDonald, L Clifford
• Lutgring, Joseph D

Titel

Reference Susceptibility Testing and Genomic Surveillance of Clostridioides difficile, United States, 2012–17

Ist Teil von

• Clinical infectious diseases, 2023-03, Vol.76 (5), p.890-896

Ort / Verlag

US: Oxford University Press

Erscheinungsjahr

2023

Link zum Volltext

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Abstract Background Antimicrobial susceptibility testing (AST) is not routinely performed for Clostridioides difficile and data evaluating minimum inhibitory concentrations (MICs) are limited. We performed AST and whole genome sequencing (WGS) for 593 C. difficile isolates collected between 2012 and 2017 through the Centers for Disease Control and Prevention's Emerging Infections Program. Methods MICs to 6 antimicrobial agents (ceftriaxone, clindamycin, meropenem, metronidazole, moxifloxacin, and vancomycin) were determined using the reference agar dilution method according to Clinical and Laboratory Standards Institute guidelines. Whole genome sequencing was performed on all isolates to detect the presence of genes or mutations previously associated with resistance. Results Among all isolates, 98.5% displayed a vancomycin MIC ≤2 μg/mL and 97.3% displayed a metronidazole MIC ≤2 μg/mL. Ribotype 027 (RT027) isolates displayed higher vancomycin MICs (MIC50: 2 μg/mL; MIC90: 2 μg/mL) than non-RT027 isolates (MIC50: 0.5 μg/mL; MIC90: 1 μg/mL) (P < .01). No vanA/B genes were detected. RT027 isolates also showed higher MICs to clindamycin and moxifloxacin and were more likely to harbor associated resistance genes or mutations. Conclusions Elevated MICs to antibiotics used for treatment of C. difficile infection were rare, and there was no increase in MICs over time. The lack of vanA/B genes or mutations consistently associated with elevated vancomycin MICs suggests there are multifactorial mechanisms of resistance. Ongoing surveillance of C. difficile using reference AST and WGS to monitor MIC trends and the presence of antibiotic resistance mechanisms is essential. Reference susceptibility testing revealed that elevated minimum inhibitory concentrations (MICs) to vancomycin and metronidazole were rare among US Clostridioides difficile isolates. Ribotype 027 was associated with increased MICs to several antibiotics including fluoroquinolones and clindamycin.