Details

Autor(en) / Beteiligte

• Kadlecová, Zuzana
• Sevriugina, Veronika
• Lysáková, Klára
• Rychetský, Matěj
• Chamradová, Ivana
• Vojtová, Lucy

Titel

Liposomes Affect Protein Release and Stability of ITA-Modified PLGA–PEG-PLGA Hydrogel Carriers for Controlled Drug Delivery

Ist Teil von

• Biomacromolecules, 2024-01, Vol.25 (1), p.67-76

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2024

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Fat grafting, a key regenerative medicine technique, often requires repeat procedures due to high-fat reabsorption and volume loss. Addressing this, a novel drug delivery system uniquely combines a thermosensitive, FDA-approved hydrogel (itaconic acid-modified PLGA-PEG-PLGA copolymer) with FGF2-STAB, a stable fibroblast growth factor 2 with a 21-day stability, far exceeding a few hours of wild-type FGF2’s stability. Additionally, the growth factor was encapsulated in “green” liposomes prepared via the Mozafari method, ensuring pH protection. The system, characterized by first-order FGF2-STAB release, employs green chemistry for biocompatibility, bioactivity, and eco-friendliness. The liposomes, with diameters of 85.73 ± 3.85 nm and 68.6 ± 2.2% encapsulation efficiency, allowed controlled FGF2-STAB release from the hydrogel compared to the unencapsulated FGF2-STAB. Yet, the protein compromised the carrier’s hydrolytic stability. Prior tests were conducted on model proteins human albumin (efficiency 80.8 ± 3.2%) and lysozyme (efficiency 81.0 ± 2.7%). This injectable thermosensitive system could advance reconstructive medicine and cosmetic procedures.