Journal of bacteriology, 1998-09, Vol.180 (17), p.4734-4738
1998
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Cloning, expression, and catabolite repression of a gene encoding beta-galactosidase of Bacillus megaterium ATCC 14581
- Ist Teil von
- Journal of bacteriology, 1998-09, Vol.180 (17), p.4734-4738
- Ort / Verlag
- United States: American Society for Microbiology
- Erscheinungsjahr
- 1998
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- A gene encoding beta-galactosidase, designated mbgA, was isolated from Bacillus megaterium ATCC 14581. Chromosomal beta-galactosidase production could be dramatically induced by lactose but not by isopropyl-beta-D-thiogalactopyranoside (IPTG) and was subject to catabolite repression by glucose. Disruption of mbgA in the B. megaterium chromosome resulted in loss of lactose-inducible beta-galactosidase production. A 27-bp inverted repeat was found to overlap the mbgA promoter sequence. Two partially overlapping catabolite-responsive elements (CREs) were identified within the inverted repeat. Base substitutions within CRE-I and/or CRE-II caused partial relief from catabolite repression. The results suggest that the 27-bp inverted repeat may serve as a target for a catabolite repressor(s).
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9193eISSN: 1098-5530DOI: 10.1128/JB.180.17.4734-4738.1998Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_107490
- Format
- –
- Schlagworte
- Bacillus megaterium, Bacillus megaterium - enzymology, Bacillus megaterium - genetics, Bacteria, Bacteriology, Base Sequence, beta-Galactosidase - genetics, Biochemistry, Cloning, Cloning, Molecular, DNA Primers, Genetics, Genetics and Molecular Biology, Glucose - metabolism, Isopropyl Thiogalactoside - metabolism, Lactose - metabolism, Mutation, Promoter Regions, Genetic, Recombinant Fusion Proteins - genetics, Transcription, Genetic