Details

Autor(en) / Beteiligte

• Hunter, E F
• McKinney, R M
• Maddison, S E
• Cruce, D D

Titel

Double-staining procedure for the fluorescent treponemal antibody absorption (FTA-ABS) test

Ist Teil von

• British journal of venereal diseases, 1979-04, Vol.55 (2), p.105-108

Ort / Verlag

England: BMJ Publishing Group Ltd

Erscheinungsjahr

1979

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The fluorescent treponemal antibody absorption (FTA-ABS) double-staining procedure was reproducible, comparable to the conventional test, and easy to read. We recommend the use of the FTA-ABS double-staining procedure for microscopes with incident illumination, the 100 x/1.30 oil achromatic objective and the 6.3 x ocular to obtain optimal fluorescence, and the KP560 as a barrier filter to exclude rhodamine emission when fluorescein fluorescence is read. With this system, errors related to poor focusing or failure to visualise treponemes on all smears should be eliminated.