Details

Autor(en) / Beteiligte

• Lei, Ye
• Shen, Hong-Fen
• Li, Qi-Wen
• Yang, Sheng
• Xie, Hong-Ting
• Li, Xu-Feng
• Chen, Mei-Ling
• Xia, Jia-Wei
• Wang, Sheng-Chun
• Dai, Guan-Qi
• Zhou, Ying
• Li, Ying-Chun
• Huang, Shi-Hao
• He, Dan-Hua
• Zhou, Zhi-Hao
• Cong, Jin-Ge
• Lin, Xiao-Lin
• Lin, Tao-Yan
• Wu, Ai-Bing
• Xiao, Dong
• Xiao, Sheng-Jun
• Zhang, Xin-Ke
• Jia, Jun-Shuang

Titel

Hairy gene homolog increases nasopharyngeal carcinoma cell stemness by upregulating Bmi-1

Ist Teil von

• Aging (Albany, NY.), 2023-05, Vol.15 (10), p.4391-4410

Ort / Verlag

United States: Impact Journals

Erscheinungsjahr

2023

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• B-cell-specific Moloney murine leukemia virus integration site 1 (Bmi-1) is overexpressed in various cancer types. We found that mRNA levels were elevated in nasopharyngeal carcinoma (NPC) cell lines. In immunohistochemical analyses, high Bmi-1 levels were observed in not only 5 of 38 non-cancerous nasopharyngeal squamous epithelial biopsies, but also in 66 of 98 NPC specimens (67.3%). High Bmi-1 levels were detected more frequently in T3-T4, N2-N3 and stage III-IV NPC biopsies than in T1-T2, N0-N1 and stage I-II NPC samples, indicating that Bmi-1 is upregulated in advanced NPC. In 5-8F and SUNE1 NPC cells, stable depletion of Bmi-1 using lentiviral RNA interference greatly suppressed cell proliferation, induced G1-phase cell cycle arrest, reduced cell stemness and suppressed cell migration and invasion. Likewise, knocking down Bmi-1 inhibited NPC cell growth in nude mice. Both chromatin immunoprecipitation and Western blotting assays demonstrated that Hairy gene homolog (HRY) upregulated Bmi-1 by binding to its promoter, thereby increasing the stemness of NPC cells. Immunohistochemistry and quantitative real-time PCR analyses revealed that HRY expression correlated positively with Bmi-1 expression in a cohort of NPC biopsies. These findings suggested that HRY promotes NPC cell stemness by upregulating Bmi-1, and that silencing Bmi-1 can suppress NPC progression.