Details

Autor(en) / Beteiligte

• Zhang, Juanye
• Ning, Yingying
• Zhu, Hua
• Rotile, Nicholas J
• Wei, He
• Diyabalanage, Himashinie
• Hansen, Eric C
• Zhou, Iris Y
• Barrett, Stephen C
• Sojoodi, Mozhdeh
• Tanabe, Kenneth K
• Humblet, Valerie
• Jasanoff, Alan
• Caravan, Peter
• Bawendi, Moungi G

Titel

Fast detection of liver fibrosis with collagen-binding single-nanometer iron oxide nanoparticles via T1-weighted MRI

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2023-05, Vol.120 (18), p.e2220036120-e2220036120

Ort / Verlag

National Academy of Sciences

Erscheinungsjahr

2023

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• SNIO-CBP, a single-nanometer iron oxide (SNIO) nanoparticle functionalized with a type I collagen-binding peptide (CBP), was developed as a T1-weighted MRI contrast agent with only endogenous elements for fast and noninvasive detection of liver fibrosis. SNIO-CBP exhibits 6.7-fold higher relaxivity compared to a molecular gadolinium-based collagen-binding contrast agent CM-101 on a per CBP basis at 4.7 T. Unlike most iron oxide nanoparticles, SNIO-CBP exhibits fast elimination from the bloodstream with a 5.7 min half-life, high renal clearance, and low, transient liver enhancement in healthy mice. We show that a dose of SNIO-CBP that is 2.5-fold lower than that for CM-101 has comparable imaging efficacy in rapid (within 15 min following intravenous injection) detection of hepatotoxin-induced liver fibrosis using T1-weighted MRI in a carbon tetrachloride-induced mouse liver injury model. We further demonstrate the applicability of SNIO-CBP in detecting liver fibrosis in choline-deficient L-amino acid-defined high-fat diet mouse model of nonalcoholic steatohepatitis. These results provide a platform with potential for the development of high relaxivity, gadolinium-free molecular MRI probes for characterizing chronic liver disease.