Details

Autor(en) / Beteiligte

• Loy, Conor J.
• Sotomayor-Gonzalez, Alicia
• Servellita, Venice
• Nguyen, Jenny
• Lenz, Joan
• Bhattacharya, Sanchita
• Williams, Meagan E.
• Cheng, Alexandre P.
• Bliss, Andrew
• Saldhi, Prachi
• Brazer, Noah
• Streithorst, Jessica
• Suslovic, William
• Hsieh, Charlotte J.
• Bahar, Burak
• Wood, Nathan
• Foresythe, Abiodun
• Gliwa, Amelia
• Bhakta, Kushmita
• Perez, Maria A.
• Hussaini, Laila
• Anderson, Evan J.
• Chahroudi, Ann
• Delaney, Meghan
• Butte, Atul J.
• DeBiasi, Roberta L.
• Rostad, Christina A.
• De Vlaminck, Iwijn
• Chiu, Charles Y.

Titel

Nucleic acid biomarkers of immune response and cell and tissue damage in children with COVID-19 and MIS-C

Ist Teil von

• Cell reports. Medicine, 2023-06, Vol.4 (6), p.101034-101034, Article 101034

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2023

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Differential host responses in coronavirus disease 2019 (COVID-19) and multisystem inflammatory syndrome in children (MIS-C) remain poorly characterized. Here, we use next-generation sequencing to longitudinally analyze blood samples from pediatric patients with COVID-19 or MIS-C across three hospitals. Profiling of plasma cell-free nucleic acids uncovers distinct signatures of cell injury and death between COVID-19 and MIS-C, with increased multiorgan involvement in MIS-C encompassing diverse cell types, including endothelial and neuronal cells, and an enrichment of pyroptosis-related genes. Whole-blood RNA profiling reveals upregulation of similar pro-inflammatory pathways in COVID-19 and MIS-C but also MIS-C-specific downregulation of T cell-associated pathways. Profiling of plasma cell-free RNA and whole-blood RNA in paired samples yields different but complementary signatures for each disease state. Our work provides a systems-level view of immune responses and tissue damage in COVID-19 and MIS-C and informs future development of new disease biomarkers. [Display omitted] •Patients with MIS-C and COVID-19 have distinct cell injury and host response profiles•Patients with MIS-C and COVID-19 have elevated solid-organ-derived cell-free DNA•Patients with MIS-C have increased endothelium- and neuron-derived cell-free RNA•Cell-free and whole-blood RNA provide complementary measurements of patient health Loy et al. use cell-free RNA, whole-blood RNA, and cell-free DNA sequencing to characterize distinct host response and cellular injury profiles in pediatric patients with MIS-C and/or COVID-19. This study highlights the complementary information from cell-free and whole-blood RNA analyses, with broad implications for future liquid biopsy applications.