Details

Autor(en) / Beteiligte

• Aikoh, T
• Tomokuni, A
• Matsukii, T
• Hyodoh, F
• Ueki, H
• Otsuki, T
• Ueki, A

Titel

Activation-induced cell death in human peripheral blood lymphocytes after stimulation with silicate in vitro

Ist Teil von

• International journal of oncology, 1998-06, Vol.12 (6), p.1355

Ort / Verlag

Greece

Erscheinungsjahr

1998

Quelle

MEDLINE

Beschreibungen/Notizen

• Silica and related substances such as silicate have been proven to possess "adjuvant effects". We have previously reported a finding of polyclonal human T cell activation induced by silicate as a superantigen in vitro. In this study, we observed activation-induced cell death in human lymphocytes after stimulation with chrysotile, a kind of silicate. Apoptotic cells were detected flow cytometrically using the TUNEL assay, and the maximum appearance of TUNEL positive cells occurred on day 4 of incubation. Simultaneously the manifestation of small-sized cells in the specimens increased implying apoptosis. Fas expression on lymphocytes increased to day 3 of incubation with chrysotile, and then spontaneously decreased on day 4 when remarkable apoptosis could be detected. Based on these results it is conceivable that activation-induced cell death occurred through Fas-Fas ligand interaction in lymphocytes after stimulation with silicate in a concentration with which no acute cytotoxicity has been detected. Whether and how the repeated apoptosis in definite clones of lymphocytes causes the induction of sFas synthesis need clarification.