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Regulation of red blood cell filterability by Ca2+ influx and cAMP-mediated signaling pathways
Ist Teil von
American Journal of Physiology: Cell Physiology, 1997-12, Vol.273 (6), p.C1828
Ort / Verlag
United States
Erscheinungsjahr
1997
Quelle
MEDLINE
Beschreibungen/Notizen
Nippon Telegraph and Telephone Corporation Medical Research
Institute, Kanto Teishin Hospital, Tokyo 141; and Department of
Physiology, Nippon Medical School, Tokyo 113, Japan
To investigate the mechanism of the
regulation of human red blood cell deformability, we examined the
deformability under mechanical stress. Washed human red blood cells
were rapidly injected through a fine needle, and their filterability
was measured using a nickel mesh filter. The decrease in filterability
showed a V-shaped curve depending on the extracellular
Ca 2+ concentration; the maximum
decrease was achieved at ~50 µM. The decreased filterability was
accompanied by no change in cell morphology and cell volume, indicating
that the decrease in filterability can be ascribed to alterations of
the membrane properties. Ca 2+
entry blockers (nifedipine and felodipine) inhibited the impairment of
filterability under mechanical stress. Prostaglandins
E 1 and E 2 , epinephrine, and
pentoxifylline, which are thought to modulate the intracellular
adenosine 3',5'-cyclic monophosphate (cAMP) level of red
blood cells, improved or worsened the impaired filterability according
to their expected actions on the cAMP level of the cells. These results
strongly suggest that the membrane properties regulating red blood cell
deformability are affected by the signal transduction system, including
Ca 2+ -dependent and cAMP-mediated
signaling pathways.
calcium entry blockers, prostaglandins, epinephrine,
pentoxifylline; adenosine 3',5'-cyclic monophosphate; calcium ion