Details

Autor(en) / Beteiligte

• Kovalenko, O V
• Golub, E I
• Bray-Ward, P
• Ward, D C
• Radding, C M

Titel

A novel nucleic acid-binding protein that interacts with human rad51 recombinase

Ist Teil von

• Nucleic acids research, 1997-12, Vol.25 (24), p.4946-4953

Ort / Verlag

England

Erscheinungsjahr

1997

Quelle

MEDLINE

Beschreibungen/Notizen

• Using the yeast two-hybrid system, we isolated a cDNA encoding a novel human protein, named Pir51, that strongly interacts with human Rad51 recombinase. Analysis in vitro confirmed the interaction between Rad51 and Pir51. Pir51 mRNA is expressed in a number of human organs, most notably in testis, thymus, colon and small intestine. The Pir51 gene locus was mapped to chromosome 12p13.1-13. 2 by fluorescence in situ hybridization. The Pir51 protein was expressed in Escherichia coli and purified to near homogeneity. Biochemical analysis shows that the Pir51 protein binds both single- and double-stranded DNA, and is capable of aggregating DNA. The protein also binds RNA. The Pir51 protein may represent a new member of the multiprotein complexes postulated to carry out homologous recombination and DNA repair in mammalian cells.