Details

Autor(en) / Beteiligte

• Balayeva, N M
• Eremeeva, M E
• Ignatovich, V F
• Rudakov, N V
• Reschetnikova, T A
• Samoilenko, I E
• Yastrebov, V K
• Raoult, D

Titel

Biological and genetic characterization of Rickettsia sibirica strains isolated in the endemic area of the north Asian tick typhus

Ist Teil von

• The American journal of tropical medicine and hygiene, 1996-12, Vol.55 (6), p.685

Ort / Verlag

United States

Erscheinungsjahr

1996

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction-amplified gene fragments was used to characterize 24 isolates of spotted fever group rickettsiae previously identified as Rickettsia sibirica from their serologic properties. These strains were obtained in Russia between 1946 and 1991 from humans and different species of Ixodid ticks. The RFLP analysis was performed using amplified DNA products obtained with a genus-specific primer pair derived from the R. prowazekii citrate synthase gene and two group-specific primer pairs from the R. rickettsii 190-kD and 120-kD surface protein antigen genes followed by Alu I, Pst I, and Rsa I restriction endonuclease digestions. Although some differences were detected in biological characteristics among the examined strains, only a single R. sibirica genotype was found with these molecular tools of identification.