Details

Autor(en) / Beteiligte

• Nègre, D
• Bonod-Bidaud, C
• Oudot, C
• Prost, J F
• Kolb, A
• Ishihama, A
• Cozzone, A J
• Cortay, J C

Titel

DNA flexibility of the UP element is a major determinant for transcriptional activation at the Escherichia coli acetate promoter

Ist Teil von

• Nucleic acids research, 1997-02, Vol.25 (4), p.713-718

Ort / Verlag

England

Erscheinungsjahr

1997

Quelle

MEDLINE

Beschreibungen/Notizen

• The specific interaction of the upstream element-containing promoter of the Escherichia coli acetate operon with either the RNA polymerase holoenzyme or its alpha subunit has been analyzed by the base removal method. Our results indicate that: (i) direct and specific base contacts can be detected in the acetate promoter-alpha subunit complex; (ii) base elimination in the upstream element of the acetate promoter enhances the binding of RNA polymerase. A similar effect is observed when studying the interactions between RNA polymerase and the rrnB ribosomal operon P1 promoter.