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Role of weight-bearing function on expression of myosin isoforms during regeneration of rat soleus muscles
Ist Teil von
American Journal of Physiology: Cell Physiology, 1996-03, Vol.270 (3), p.C763-C771
Ort / Verlag
United States
Erscheinungsjahr
1996
Quelle
MEDLINE
Beschreibungen/Notizen
X. A. Bigard, D. Merino, B. Serrurier, F. Lienhard, Y. C. Guezennec, K. J. Bockhold and R. G. Whalen
Departement de Physiologie Systemique, Centre d'Etudes et de Recherchesde Medecine Aerospatiale, Bretigny-sur-Orge, France.p
The expression of myosin isoforms was studied in regenerated rat soleus
muscle during either normal or altered postural activity. Regeneration was
induced following injury by venom from the Notechis scutatus scutatus
snake. Immunohistochemical analysis showed that, in regenerating soleus
muscle after 3 wk of hindlimb suspension, nearly all fibers reacted
positively with the myosin heavy chain (MHC) antibody associated with
fast-twitch muscle fibers (fast MHC). When 3 wk of recovery with normal
weight-bearing activity followed hindlimb suspension, the regeneration
soleus muscle exhibited a nearly homogeneous staining with the MHC antibody
associated with the slow-twitch muscle fibers (slow MHC). These findings
were in accordance with quantitative analysis of the electrophoretic
separation of the native myosin isoforms. Immunohistochemical data showed
that removal of weight bearing in the 21-day old regenerated soleus muscles
resulted in an increase in fast MHC expression. Together, the results of
the present study clearly demonstrate that the postural load is an
important component in the induction of slow MHC in regenerating muscle and
that the control of the expression of MHC in muscle comprising a
homogeneous population of fibers deriving from satellite cells appears more
homogeneous and more complete than in a nondegenerated one.