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Using the method of electrophoretic separation of isoenzymes of serum alkaline phosphatase the authors confirmed the diagnosis of transient hyperphosphatasaemia in nine children examined in the out-patient department or hospitalized with various diagnoses in the Childrens Hospital. The typical isoenzyme picture was formed by an atypical anodal fraction and the probable bone isoenzyme corresponding to the cathodal fraction. The examination method used made it possible to differentiate rapidly and relatively simply transient hyperphosphatasaemia from other diseases associated with a high serum alkaline phosphatase activity.