Details

Autor(en) / Beteiligte

• Teare, J P
• Punchard, N A
• Powell, J J
• Lumb, P J
• Mitchell, W D
• Thompson, R P

Titel

Automated spectrophotometric method for determining oxidized and reduced glutathione in liver

Ist Teil von

• Clinical chemistry (Baltimore, Md.), 1993-04, Vol.39 (4), p.686-689

Ort / Verlag

England

Erscheinungsjahr

1993

Quelle

MEDLINE

Beschreibungen/Notizen

• An enzymatic recycling method has been applied to the measurement of total and oxidized glutathione with a centrifugal analyzer. When the reduced form of glutathione (GSH) was masked with 2-vinylpyridine to measure the oxidized glutathione (GSSG), the time to ensure full derivatization was three times longer than has been reported. The method is quick, simple, accurate, and precise (1.27% for GSH, 3.3% for GSSG intraassay CV; 2.15% for GSH, 5% for GSSG interassay CV), and the automation allows large numbers of samples to be conveniently assayed.