Details

Autor(en) / Beteiligte

• Vinter, V G
• Zotkina, N L
• Zo, S Kh
• Abramova, Z I

Titel

Purification and immunochemical characteristics of neutral Mn-dependent DNAase of chromatin

Ist Teil von

• Biokhimiia (Moscow, Russia), 1993-08, Vol.58 (9), p.1394

Ort / Verlag

Russia (Federation)

Erscheinungsjahr

1993

Quelle

MEDLINE

Beschreibungen/Notizen

• A scheme for purification of neutral Mn-dependent chromatin DNAase has been developed. The two-step purification procedure, which includes chromatography on hydroxyapatite and isoelectrofocusing, allows one to obtain preparative amounts of the enzyme having a specific activity of more than 3000 u./mg, molecular mass of 41 kDa and pI of 9.2.