Proceedings of the National Academy of Sciences - PNAS, 1994-07, Vol.91 (15), p.7321-7325
1994
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Details
- Autor(en) / Beteiligte
- Titel
- The Bifunctional Iron-Responsive Element Binding Protein/Cytosolic Aconitase: The Role of Active-Site Residues in Ligand Binding and Regulation
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 1994-07, Vol.91 (15), p.7321-7325
- Ort / Verlag
- United States: National Academy of Sciences of the United States of America
- Erscheinungsjahr
- 1994
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The iron-responsive element binding protein/cytosolic aconitase functions as either an RNA binding protein that regulates the uptake, sequestration, and utilization of iron or an enzyme that interconverts citrate and isocitrate. These mutually exclusive functions are regulated by changes in cellular iron levels. By site-directed mutagenesis we show that (i) ligation of a [4Fe-4S] cluster is necessary to inactivate RNA binding and activate enzyme function in vivo, (ii) three of four arginine residues of the aconitase active site participate in RNA binding, and (iii) aconitase activity is not required for iron-mediated regulation of RNA binding.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.91.15.7321Titel-ID: cdi_pubmed_primary_8041788
- Format
- –
- Schlagworte
- Aconitate Hydratase - metabolism, Animals, Binding Sites, Cell Line, Cell lines, Citrates, Cloning, Molecular, Cytosol - enzymology, Enzymes, Gels, Gene expression regulation, Genetic mutation, HeLa cells, Iron - metabolism, Iron regulatory proteins, Ligands, Ligation, Mice, Mutagenesis, Site-Directed, RNA, RNA, Messenger - metabolism, RNA-Binding Proteins - genetics, RNA-Binding Proteins - metabolism