Details

Autor(en) / Beteiligte

• Khettal, B
• de Lauzon, S
• Desfosses, B
• Ushida, S
• Marquet, A

Titel

A catalytic antibody isomerizing a delta 5-3-cétostéroïde

Ist Teil von

• Comptes rendus de l'Académie des sciences, Série III, Sciences de la vie, 1994-05, Vol.317 (5), p.381

Ort / Verlag

France

Erscheinungsjahr

1994

Quelle

MEDLINE

Beschreibungen/Notizen

• Antibodies were raised against 3-fluoro-4-aza-estradiol-17-hemisuccinate to elicit abzymes capable of isomerizing delta 5-3-ketosteroids. The hapten was designed to present a planar A ring showing some analogy with the intermediate dienol and a polarity capable of inducing catalytic groups. Antibodies binding the hapten tightly were cloned and purified from either ascites or hybridoma supernatants. Catalytic activity was tested with androst-5-ene-3,17-dione. Among the five different monoclonal antibodies binding the hapten, one proved to enhance significantly the substrate isomerization rate (kcat/kuncat = 830). Initial rates followed Michaelis kinetics and the activity was competitively inhibited by the hapten.