Details

Autor(en) / Beteiligte

• Klukas, O
• Peshenko, I A
• Rodionov, I L
• Teliakova, O V
• Utkin, Iu N
• Tsetlin, V I

Titel

Fragments 183-198 and 125-145 of the alpha-subunits of the Torpedo californica nicotinic acetylcholinergic receptor binds alpha-bungarotoxin and neurotoxin II from Naja naja oxiana

Ist Teil von

• Bioorganicheskaia khimiia, 1995-02, Vol.21 (2), p.152

Ort / Verlag

Russia (Federation)

Erscheinungsjahr

1995

Quelle

MEDLINE

Beschreibungen/Notizen

• Interaction of the mono[125I]iodinated alpha-bungarotoxin and neurotoxin II Naja naja oxiana with the synthetic peptides corresponding to the fragments of the alpha-subunit of nicotinic acetylcholine receptor from Torpedo californica was studied. It was found that both toxins bind to the fragments alpha 186-198, alpha 183-198 and alpha 125-145 adsorbed to the 96-well P.E.T.G. assay plates (COSTAR). Acm-groups on Cys residues did not prevent toxin binding by the peptides studied. Determination of the binding parameters showed that alpha-bungarotoxin interacts with fragment alpha 125-145 less effectively than neurotoxin II. The data obtained demonstrate the presence of different toxin-binding sites on alpha-subunit and confirm the model of multipoint neurotoxin-receptor interaction.