Details

Autor(en) / Beteiligte

• Corsi, P
• Gennarini, G
• Ruccia, D
• Vitiello, F
• Di Benedetta, C

Titel

Analysis of proteins and glycoproteins from adult bovine cerebellum. II. Quantitative separation by Con A Sepharose chromatography

Ist Teil von

• Bollettino della Societa italiana di biologia sperimentale, 1980-05, Vol.56 (10), p.1071

Ort / Verlag

Italy

Erscheinungsjahr

1980

Quelle

MEDLINE

Beschreibungen/Notizen

• Soluble and insoluble glycoproteins from adult bovine cerebellum have been separated by affinity chromatography on ConA-Sepharose and analyzed by polyacrylamide gel electrophoresis I2% in presence of SDS. Soluble fraction represents 26% of total proteins. Within soluble and insoluble fractions 4.5% of proteins binds to ConA. Electropherograms of the soluble and insoluble fractions as well as of the proteins not absorbed on ConA-Sepharose, display a very complex pattern. Soluble fractions present many sharp bands in the region of molecular weight above 100 k. Heterogeneity is lesser in ConA-binding proteins. The contamination by Concanavalin A is considered. At present no definite conclusions can be drawn regarding the similarities existing between s.c. soluble and membrane-bound cerebral glycoproteins.