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The atomic absorption spectrophotometer AAS1 was used to determine the concentration of Zn and Mn in the spermatozoa and ejaculate plasma of rams, having different percent of motile spermatozoa. A reverse correlation was found between the Zn and Mn content of spermatozoa and the percent of their motility. Seminal fluid with a higher percent of motile cells had plasma with a higher Zn and Mn content as compared with ejaculates having plasma with a lower percent of motile spermatozoa.