Details

Autor(en) / Beteiligte

• Sukhomlinov, B F
• Fedorovich, A N
• Chaĭka, Ia P

Titel

Method of isolation of intact enterocytes from rat duodenal mucosa

Ist Teil von

• Ukrainskij biohimičeskij žurnal, 1983-03, Vol.55 (2), p.199

Ort / Verlag

Ukraine

Erscheinungsjahr

1983

Quelle

MEDLINE

Beschreibungen/Notizen

• A procedure is suggested for obtaining intact enterocytes from rat duodenum mucosa by the tissue trypsinization in the isotonic Krebs solution (pH 7.9). The maximum yield of certain enterocytes is reached at the enzyme concentration of 0.03%; the incubation time--15 min; 200-250 mg of mucosa produces 3,7 X 10(6)--4,7 X 10(6) cells.